What kind of connection exists between Rf and retention of the solute?

What kind of connection exists between Rf and retention of the solute?

There exists a inverse narration between Retention time of the solute Rf  which can be described as follows:

 

If reservation time of the solute increases than the value of Rf  factor decreases and if the retention time of the solute decreases than the value of Rf  factor increases”.

Discrepancy of factor “R”

The value of R may commute with following factors:

Kind of movement

Sorbent and solvent

Concentration

These play a little role in the change of R factor.

Now question arises that how value of R decreases?

“The value of the factor R usually decreases when concentration of the solute in the process of adsorption of the solute”.

Replication of R factor within Experimental process in controlled conditions

In addition,when we deal with experimental procedure R value can be reproduced if following conditions are controlled:

Solvent

Sorbent

Porosity

Concentration of the solute

Temperature

Resolution

Definition

“Difference between two different zones centers is known as resolution”

Resolution factor is another factor which is included within the basic concepts of Chromatography.

How resolution factor is important in the effective separation?

Distance between two centers of two different zones as the rate of movement

Compactness of zones

What is the Effect of resolution factor on separation?

“Distance between two centers of two different zones as the rate of movement”

This factor has direct relation on separation which is described as follows:

Larger distance:

If distance between two different centers of two different zones increases than separation will be more effective.

Smaller distance:

If distance between two different zones centers is smaller or decreases than separation will be poor and will be not effective.

 

 

 

 

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