What do you know about the process of partition in chromatography?
Partition process can be defined as
“The process which causes the division or separation of two components”
In the terms of chromatography partition chromatography is greatly difficult as compared to other types of chromatography.Partition methods are slightly complicated as compared to other process of chromatography.
Need of suitable support
A suitable support is needed within a large surface area on which stationary phase is to be hold it.Support should be of one kind either polar or non-polar
Partition method is considered as old method among all the methods of chromatography.
Advantages and limitations of partition methods
Large resolving power
Partition method provides a large resolving power than adsorption methods because it is slightly difficult to proceed.In this process it is difficult to achieve the relation between stationary phase and mobile phase when both are in liquid state.
More suitable method
Partition method is a suitable method for such mixtures which have low concentrations.
(recent advances have been overcome method provide to this process)
Partition method is dependent method is dependent on solubility between two liquids small difference in molecular weight will affect the partition method therefore partition method is slightly suitable for the separation of homologous series.
Partition coefficient is slightly independent on concentration over a great variety of compounds.partition method provide a linear isotherm over a wide range of concentration.
This method appears to be a more rational relational structure and substitution method affect the partition methods.