What do you know about the process of adsorption in chromatography?

What do you know about the process of adsorption in chromatography?

Definition of adsorption

Adsorption process can be defined as

“The process which holds the molecules of solid together on the surface of the solute”.

Role of adsorption in effective separation

Commonly adsorption chromatography is slightly difficult to carryout as compared to other chromatography.However in general two other factors which must be considered in the process of adsorption

Types of different compounds which are to be separated

For which purpose separation is to be done

Enlist some process which are involved in the process of adsorption?

Although adsorption process is a complicated process ,but this mechanism involve the following factors

A single type of polarity

Stands out.

General discussion on column chromatography

In general column chromatography can be slightly reliable on above discussed factor which can give following results:

Reliable predictions of suitable support

Mobile phase

Stationary phase

 

Advantages and limitations of adsorption

Awareness in adsorption process facilitate the selection of suitable technique for the particular separation process .

Advantages of adsorption

Adsorption process are experimentally easier.

Adsorption provides a more uniform,reproducible behaviour

Because a single eluting mobile phase is involved in this process

Adsorption process is slightly sensitive to steric factor for similar molecules and it is therefore applicable to the separation of such kinds of molecules

Polarity of the mobile phase can be changed at a great level because miscibility of stationary phase in a liquid state is not involved.

Adsorption chromatography is suitable for the separation of a great variety of compounds

Adsorption chromatography is suitable for the separation of components of mixtures whose components are widely distributed according to the polarity or their structure.

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