What do you know about the process of adsorption in chromatography?
Definition of adsorption
Adsorption process can be defined as
“The process which holds the molecules of solid together on the surface of the solute”.
Role of adsorption in effective separation
Commonly adsorption chromatography is slightly difficult to carryout as compared to other chromatography.However in general two other factors which must be considered in the process of adsorption
Types of different compounds which are to be separated
For which purpose separation is to be done
Enlist some process which are involved in the process of adsorption?
Although adsorption process is a complicated process ,but this mechanism involve the following factors
A single type of polarity
General discussion on column chromatography
In general column chromatography can be slightly reliable on above discussed factor which can give following results:
Reliable predictions of suitable support
Advantages and limitations of adsorption
Awareness in adsorption process facilitate the selection of suitable technique for the particular separation process .
Advantages of adsorption
Adsorption process are experimentally easier.
Adsorption provides a more uniform,reproducible behaviour
Because a single eluting mobile phase is involved in this process
Adsorption process is slightly sensitive to steric factor for similar molecules and it is therefore applicable to the separation of such kinds of molecules
Polarity of the mobile phase can be changed at a great level because miscibility of stationary phase in a liquid state is not involved.
Adsorption chromatography is suitable for the separation of a great variety of compounds
Adsorption chromatography is suitable for the separation of components of mixtures whose components are widely distributed according to the polarity or their structure.