What do you know about some underlying principles of chromatography discuss one of them in detail?

What do you know about some underlying principles of chromatography discuss one of them in detail?

What do you know about some underlying principles of chromatography discuss one of them in detail?

Different basic principles are given as follows

Distribution value

Resolution value

Peak shape

Efficiency

Here Distribution value is discussed in detail as follows:

Distribution value Distribution value can be defined as follows

“Relationship which explains the relationship between concentration of solute maintained by one phase and attached to another phase”.

General representation of distribution factor

Distribution value can be represented as follows

Symbolic representation

K=distribution factor

K=C1/C2

C1=concetration of the solute in phase 1

C2=Concentration of the solute in phase 2

Modification of distribution equation

This equation can be limited to a someone special type of chromatography technique.

Distribution factor is dependent upon

Temperature

Pressure

Distribution value is independent upon the concentration.

However in practice distribution value is independent upon amount of concentration in certain cases.It Is seen over a limited range of chromatography technique.

Discuss the Distribution value in case of partition method?

In partition method distribution co-efficient is called partition method.This also applies in situation with solvent extraction.

In this case

C1 is changed into concentration of stationary phase

C2 is changed into concentration of mobile phase

They are changed into Cs Cm

Cs=Concentration of the stationary phase

Cm=Concentration of the mobile phase

Kp=Cs/Cm

Kp= Distribution co efficient in case of partition co -efficient

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