What do you know about liquid mobile phase in a column method and  partition methods of chromatography?

What do you know about liquid mobile phase in a column method and  partition methods of chromatography?

Column methods are very common and generally a liquid mobile phase is used for this purpose.Ion exchange and exclusion chromatography are also used in column chromatography.

On the basis of above discussion we can say that this type of chromatography is called liquid column chromatography.

How much amount of sample is introduced in a column within liquid column chromatography?

The mixture which is to be separated is injected into a small amount within a column.best way to introduced the sample is introduction of sample at the top position of column followed by liquid mobile phase (known as elution agent).

Involvement of Adsorption process in separation

If separation is due to adsorption process than this separation will be dependent on relationship between Solute, solvent and surface of the absorbent.

Separation by a partition method

In case of partition method separation will be dependent upon division of solute among two phase liquid mobile phase and stationary liquid phase.

What are the indications of success full separations?

Success full separations are those separations in which

Dimension of column is carefully selected

Liquid mobile and stationary phase are also selected carefully.

What do you know about different terms about columns used with in column chromatography?

Columns which are used in chromatography may be different shape and designs.some type of columns are also made up of glass and chemically inert material.

Main purpose of all above different terms of column:

A column should support stationary phase and controlled flow of solvent.The length or diameter of the column should be In the range of 10 or greater than this value.

Diameter of column according to the need of mixture

In case of complex mixture large diameter of column should be made and vice versa.

 

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