What do you know about different designs of columns for effective separations?

What do you know about different designs of columns for effective separations?

In almost all cases many types of columns are made for complete and effective procedure of elution through different areas of a column.Passage of mobile phase through a column is controlled by using different physical means such as

Use of stopper or stop cock

Pinch clamp employing gravity

By using pumps

Tube system carrying the mobile phase towards the column

Effluent moving away from the column.

Precautionary measures for the use of effluent

It should be inert with the reference of mobile phase

Materials from which tube like columns are designs

Commonly used materials for making tube like columns are given in following:

Glass

Polyethylene

Teflon and e-t-c

How tube like columns with joints are made?

Jointed tubes are connected to each other through following materials

Connectors

Sleeves

Swag lock connectors

what are the precautionary measures for the designing of column?

Over designing of the column can cause the wastage of materials

Can cause the poor and in- effective separation

Large amount of mobile phase is used before the emergence of components through the column

Areas or different zones of the columns may increase their size due to occurring process of diffusion

 

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