What do you know about different designs of columns for effective separations?
In almost all cases many types of columns are made for complete and effective procedure of elution through different areas of a column.Passage of mobile phase through a column is controlled by using different physical means such as
Use of stopper or stop cock
Pinch clamp employing gravity
By using pumps
Tube system carrying the mobile phase towards the column
Effluent moving away from the column.
Precautionary measures for the use of effluent
It should be inert with the reference of mobile phase
Materials from which tube like columns are designs
Commonly used materials for making tube like columns are given in following:
Teflon and e-t-c
How tube like columns with joints are made?
Jointed tubes are connected to each other through following materials
Swag lock connectors
what are the precautionary measures for the designing of column?
Over designing of the column can cause the wastage of materials
Can cause the poor and in- effective separation
Large amount of mobile phase is used before the emergence of components through the column
Areas or different zones of the columns may increase their size due to occurring process of diffusion