What are different techniques which are used for the determination of separated components separated by the chromatography procedure?
There are different methods which are used for the separation of different components separated by the help of chromatography procedure.
In the start each component which is separated by a container which is kept separated.After this the separation of component is done by Chemical or instrumental methods.
Second fact of separation is based upon the area of the chromatography peak.
This fact can be explained as follows:
Area of the chromatography peak is directly proportional to the amount of the separated component within the peak.
Which parameters are followed for the determination of area of peak and other different factors associated to this amount?
These parameters are given as follows:
Height of the peak
Weighing of following things
Peak, disc ,Electronics digital integration
Discuss the Requirement of above given parameters?
Following things are needed for the above parameters
Initiation of sample in a suitable way
Worthy chromatography System of equilibrium along the column
Familiar and consistent rate of flow for the mobile phase
Separation of component by a factor area of the peak
Separation of the component by the help of area of the peak is given as follows
Erstwhile area is determined, standardized curves can be prepared by the help of different standardize curves.Curves are plotted within the area against concentration of the reference.The unknown component is chromatographed under same conditions of
from the standard curves.
Discuss the factors affecting the area of the peak?
Height of the peak is determined by the distance from the reference line to the top point of peak.
How best results are obtained from the area of the peak?
Efficient results are obtained by keeping following points of area of the peak
Peak should be well defined
The shape of peak should be in Gaussian shape
Peak should be resolute from the other peaks.
Height of the peak and area of the peak both are directly related to each other.
Previous condition is followed in those case when shape of the peak is triangular.
Definition of planimetery
A planimeter can be defined as follows
“The device which is used to trace the circumference of the peak”.
The result is consolidation of the peak along its direct recording of peak on the dial of the peak.