What is the best methodology for the impregnation of the support in partition chromatography?
Columns in partition chromatography are difficult to prepare because the support must retain the stationary phase .commonly the stationary phase in liquid form is introduced on the support prior to the packing of the column.
A large variety of techniques are available for the impregnation of the support and the one that will be selected will depend on the inertness of the support and the kind of stationary phase and also the amount of stationary phase in liquid phase as required.
Most common method for the mixing of the support
The most common method for the mixing of the support and the liquid intimately in a mortar and pestle or in another container.The impregnated powder should be is free flowing and not wet.It is also possible to prepare a column of the support and pass the stationary liquid phase through the column until the support is evenly coated with liquid.
Technique used in reversed phase chromatography
For reversed phase chromatography reversed phase support,a common technique is to dissolve the stationary phase in a liquid form with in volatile solvent,the support is added to this solution,and the volatile solvent is removed by evaporation leaving the support uniformly coated.
Permission of this procedure
This procedure permits an accurate control of the amount stationary phase on the support if in the start initial quantity of sample is weighed out.